HOT FIREPol® SolisGreen® qPCR Mix
Dye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate the DNA amplification process during PCR. HOT FIREPol® SolisGreen® qPCR Mix is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating SolisGreen® dye. It comprises all the components necessary, excluding the template and primers, to perform highly sensitive qPCR. The user simply needs to add water, template, and primers. HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x qPCR buffer with 12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP SolisGreen® dye Internal reference based on ROX dye SolisGreen® dye This dye-based qPCR mix contains next-generation DNA binding dye SolisGreen®. The fluorescence spectra of this dye is similar to the more widely used SYBR® Green I and is compatible with most major real-time cyclers. SolisGreen® dye shows: high fluorescence level high sensitivity for detecting low template concentrations high stability for room temperature storage